A collection of wheat seeds with more than 10 million sequenced and carefully cataloged genetic mutations is now freely available to wheat breeders and researchers around the world. The resource is already aiding in the development of wheat plants with improved traits and according to scientists, will greatly accelerate the study of gene functions in wheat.
“I think it’s really a game changer for our community,” Jorge Dubcovsky, from the Howard Hughes Medical Institute said in a recent article.
This is because wheat is a vital crop, supplying about 20% of the calories consumed by humans worldwide. To maintain food security, wheat breeders are working to develop plants that offer more nutritional value, have greater yields, and can thrive in a changing climate.
A key genetic feature makes the plant difficult to study and manipulate, however. Like many plants, wheat is polyploid, meaning it has multiple copies of its genome in every cell: Pasta wheat has two copies of every gene, and bread wheat has three. To study the function of an individual gene, researchers typically mutate or eliminate that gene to find out what happens—an approach known as reverse genetics.
But in a polyploid organism such as wheat, mutations in individual genes often have no apparent effect, because additional copies of the mutated gene compensate for the loss. Researchers explain that they must cross plants with mutations in different copies of the gene several times to obtain a generation of plants in which the gene’s function is lost. The gene copies also hide natural variation in the wheat genome that could create opportunities to selectively breed plants with useful traits.
According to the article, Dubcovsky and his colleagues chemically induced random genetic mutations in thousands of wheat seeds and began developing and characterizing their collection of wheat mutant lines more than five years ago. To make it possible to analyze the DNA of all of the lines, the researchers developed an approach that let them focus on the small fraction of the genome that encodes proteins.
Focusing on this small portion of each plant’s genome, the team sequenced 400 billion bases of DNA using sophisticated sequencing technology to analyze the plants that grew from the mutated seeds—a total of 2,735 mutant lines. Seeds from each plant line were increased and saved for distribution.
Because wheat’s polyploidy enables it to tolerate many mutations without impairing growth, the researchers were able to develop lines with a high density of genetic mutations—thousands were detected in every plant. More than 90% of the plants’ genes are disrupted by the 10 million mutations cataloged in the collection, making it a powerful resource for studying the function of nearly any wheat gene.
Researchers can search the database to find lines with mutations in the genes they want to study, then order seeds. More than 3,000 seed stocks have already been distributed to wheat researchers around the world, Dubcovsky said.
Dubcovsky stresses that the collection, which includes both bread and pasta wheats, is valuable because it makes it possible to do genetic research and manipulations directly in this economically important crop. His own team is using the collection to characterize genes that control the plant’s flowering, and has already used the wheat lines to develop wheat varieties with larger grains and more dietary fiber.
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